EpCAM antibody (FITC)

Catalog No. ABIN336890

The Mouse Monoclonal anti-EpCAM antibody has been validated for FACS, IHC and IHC (p). It is suitable to detect EpCAM in samples from Human.

Quick Overview for EpCAM antibody (FITC) (ABIN336890)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ESA214

Product Details anti-EpCAM Antibody (FITC)

Specificity: Molecular Weight: 40 kDa.

Immunogen: Small cell lung carcinoma

Isotype: IgG1 kappa

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. .(Antigen retriever like pepsin enhances the staining of FFPE tissue, please see data sheet for pepsin cat. # AR-6543. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Breast carcinoma. Cellular Localization: Cell membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The concentrated antibodies (Protein A purified) are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Epithelial is a transmembrane glycop

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM